Welcome to the Biotage Flash Purification Blogs.

    When is Gradient Elution Better than Isocratic Elution?

    March 30, 2022 at 2:00 PM / by Bob Bickler posted in isocratic, linear gradient, step gradient

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    Gradient or isocratic elution, that is the question. Within flash chromatography these are the options afforded to separate reaction mixtures and natural product extracts and is the focus of this post.

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    How should normal-phase gradient solvents be chosen?

    March 14, 2022 at 1:43 PM / by Bob Bickler posted in Solvents, Normal Phase, gradient

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    Normal-phase flash chromatography is an integral component of the organic synthesis workflow. Since reactions rarely generate 100% pure product, they need purification and flash chromatography is the most utilized tool for that task.

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