Welcome to the Biotage Flash Purification Blogs.

    What is a step gradient and why should I use it?

    September 27, 2022 at 2:07 PM / by Bob Bickler posted in gradient, step gradient


    Most chemists I meet utilizing flash chromatography use linear gradients. These gradients are either very generic (X% to Y% over a certain time or volume) or can be complex with multiple slope changes and even isocratic holds. These complex gradients are often created during a purification to get the desired separation because the generic gradient does not provide enough separation for the target compound needing purification.

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