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Bob Bickler

Isolation of some minor cannabinoids using flash chromatography

April 16, 2019 at 11:02 PM / by Bob Bickler

Cannabis entrepreneurs continually seek to differentiate themselves from others in the market. Some focus on THC while others focus on CBD. What I have seen recently after attending some cannabis-specific conferences is a growing interest in isolating/purifying some of the minor, naturally occurring phytocannabinoids such as CBG and CBDV.

In this post, I discuss some work I recently performed in my lab with a hemp extract where I separated, identified, and isolated CBG and CBDV.

 

For those needing to purify cannabis and hemp extracts in order to remove impurities and separate/isolate individual cannabinoids, one of the most successful tools used to achieve these goals is flash chromatography. For those unfamiliar with flash chromatography, it is a process that uses an automated purification system consisting of a pump, a column filled with media (either a waxy sorbent or silica), solvents (ethanol + water, or hexane + ethyl acetate), an ultraviolet detector (detects the cannabinoids as they exit the column), and a fraction collector (collects the detected compounds) – all controlled by software.

There are two useful flash chromatography modes, normal-phase and reversed-phase. Normal-phase uses a silica filled column and non-polar solvents such as hexane and ethyl acetate while reversed-phases uses a waxy, bonded silica with water and ethanol as solvents. Their separation mechanisms are different and your purification goals are will dictate which mode to use.

For instance, to remediate THC from CBD, reversed-phase flash chromatography is the best mode because it separates compounds with different hydrophobicities, Figure 1.

 


THC remediation 2

Figure 1. Reversed-phase flash chromatography removes THC from CBD and partially removes CDBV.

 
 
Normal-phase is useful when separating compounds with significantly different polarities. It is useful for removing some polar pesticides (myclobutanil, tebuconazole, etc.) and as a general clean up step to remove unwanted co-extractants like carotenoids (very hydrophobic) and polar, co-extracted impurities. Normal-phase flash chromatography, however, is not useful for separating THC from CBD as their polarity differences are not significant enough to be a useful technique, Figure 2.

 

Cannabinoid structures CBG, CDB, CBDV, THC

Figure 2. Chemical structures of THC, CBD, CBDV, and CBG account for the differences in polarity and hydrophobicity.

CBD differs from THC only in the number of -OH functional groups, CBD has two while THC contains only one which impacts hydrophobicity more than polarity. CBG has two -OH groups like CBD but lacks a second six-membered ring which impacts polarity. CBDV has fewer carbon atoms than the others impacting hydrophobicity.

What about CBG and THCV? Can the structure differences be exploited using flash chromatography? If so, which mode works best for them?

Well, we know that we can separate THC, CBD, and CBDV using reversed-phase because of hydrophobicity differences. The separation of CBD and CBG, however, using reversed-phase flash chromatography is difficult because the hydrophobicity differences are not large enough to exploit, resulting in co-elution, Figure 3.

 

Hemp 50-100 step 100 mg DLV with mass-1

Figure 3. Reversed-phase hemp distillate purification shows the separation of CBDV (+287) from the other eluting cannabinoids. However, CBD (+315) and CBG (+317) co-elute.

Since reversed-phase was unsuccessful at separating CBG from CBD, I tried normal-phase. As it turns out, CBG is separable from both CBD and THC using this flash chromatography mode because of polarity differences, even at high loads (20% of silica mass), Figure 4.

 

CBG purification NP

Figure 4. Successful purification of CBG from CBD and other cannabinoids in a hemp distillate using normal-phase flash chromatography.

I am sure by now that you have noticed the data in figures 1, 3, and 4 have numbers next to many of the “peaks”. These are the detected molecular ions of the various cannabinoids. In order to verify each UV-detected peak's identity, I used a flash chromatography system with an in-line mass detector (Biotage® Isolera Dalton 2000). This flash system simplifies flash chromatography by providing real time identification of an eluting compound’s molecular weight.

Using a flash system with an in-line mass detector is also useful when the compounds have the same molecular weight but are separated, e.g. CBD and THC, and when trying to determine the "unknowns", Figure 5.

 

Hemp minor cannabinoids NP

Figure 5. Flash chromatography with a mass detector enables identification and location of minor cannabinoids in an extract. In this hemp distillate we find four detectable masses, 329, 333, 331, and 288, which may be other cannabinoids.

These other detected compounds with masses 329, 331, 333, and 288 may also be cannabinoids. Possibilities include: cannabicoumarone (329), cannabielsoin (CBE) and/or CBDV-A (331), CBGV-A (333) or cannabichromaraone. The 288 mass does not match any cannabinoid or terpenoid that I know.

 

While an automated flash chromatography system with a mass detector is helpful, it certainly is not required for this work. In fact, methods developed on the Isolera Dalton 2000 can only be scaled-up a certain extent, in this normal-phase example, a maximum of 150 grams or milliliters of distillate per purification cycle is possible with a 750 gram silica column. 
 
So, to scale purification beyond 150 grams, you can use the identical method  (programmed in column volumes) used with the Isolera Dalton 2000 on one of our larger scale systems Biotage® Isolera LS, Biotage Selekt, or Biotage Flash 150) since you will know the number of column volumes required to elute your targeted cannabinoids.  
 

Interested in learning more about flash chromatography and cannabis? Check out our infographic available for download here:

 

Cannabis Infographic

Topics: cannabis, CBD, CBG, purify

Bob Bickler

Written by Bob Bickler

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