Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables. In previous posts I have talked about selecting the best solvents, their ratios, and maximizing load based on TLC Rf data. These are all important chromatography-generated variables but now I would like to share some tips on actual technique differences and their impact on purification performance.
In particular in this post I will focus on the benefits and drawbacks of liquid loading and dry loading. Both have their place in liquid chromatography but when should one technique be used over another?