Welcome to the Biotage Flash Purification Blogs.

      How do I purify ionizable organic amine compounds using flash column chromatography?

      November 21, 2019 at 3:59 PM / by Bob Bickler posted in Amine, Chromatography, Gradient, Normal phase, Optimization, Polar, Solvents, Troubleshooting, TLC

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      For most organic reaction mixture purifications the process is fairly straightforward. Use hexane/ethyl acetate or, for polar compounds, DCM/MeOH.  But what do you do if this doesn't work and your compounds are basic organic amines?

      In this post, I re-examine the options available to achieve an acceptable organic amine purification when typical separation methods are insufficient.

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      Is there an easy way to purify organic amines?

      April 11, 2019 at 9:35 AM / by Bob Bickler posted in Amine, Solvents

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      If you synthesize organic amine compounds, especially heterocyclic, secondary, or tertiary amines, you likely have encountered problems with their chromatography using silica columns. With the amine groups being basic and silica being acidic, there is a natural attraction between the two. This sometimes strong attraction often requires the use of a competing amine in the solvent system. Modification of the mobile phase with the addition of a solvent like triethyl amine can provide a successful purification. Often times the use of an amine-modified stationary phase can provide the needed conditions to avoid the acid-base interaction that can interfere with a successful flash chromatography purification.

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