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    Are reversed-phase flash chromatography columns designed for aqueous solvents necessary?

    December 30, 2019 at 3:54 PM / by Bob Bickler posted in Reversed-phase, Water, Aqueous


    When it comes to the purification of polar, water-soluble compounds reversed-phase chromatography is the most commonly used approach. However, because of strong stationary phase – mobile phase repulsion forces, the use of highly aqueous (90-100% water) solvent systems has been shown to provide less retention than needed.  This issue has led to the development of “aqueous compatible” reversed-phase media.

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