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    When should I use a pH modifier in flash column chromatography gradient?

    February 11, 2020 at 3:15 PM / by Bob Bickler posted in pH, acid, base


    Have you ever experienced compound tailing or streaking on your TLC plate or flash chromatography results and wondered what in the world is going on here? Well, there can be multiple reasons for this problem including poor mass-transfer kinetics, secondary solute-sorbent interactions, or unstable compound chemistry.

    In this post, I will discuss one technique that has been shown to work time and time again to address the issue.

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