Welcome to the Biotage Flash Purification Blogs.

    Why do I see more peaks than I expect with flash column chromatography?

    January 8, 2020 at 3:23 PM / by Bob Bickler posted in Chromatography, Gradient, Normal phase, Solvents, Troubleshooting

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    Are you observing more chromatographic peaks than you expect compared to TLC or other assessment data?  Well, it could be that your method is separating some isomers or, it could be that there is an actual method issue.

    In this post I will discuss what could cause a method issue and suggest some ideas as to how to fix it.

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    Green Flash Chromatography Webinar

    November 26, 2019 at 9:03 PM / by Bob Bickler posted in Chromatography, Webinar

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    Over the past several decades, the chemical industry has implemented process changes and updated practices in R&D and manufacturing in an effort to reduce liquid and solid lab waste. The pharmaceutical industry in particular has taken steps within their drug discovery labs to reduce solvent use by requiring their chemists to find and implement measures that achieve the corporate environmental goals without curtailing their productivity – quite the challenge.

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    How do I purify ionizable organic amine compounds using flash column chromatography?

    November 21, 2019 at 3:59 PM / by Bob Bickler posted in Amine, Chromatography, Gradient, Normal phase, Optimization, Polar, Solvents, Troubleshooting, TLC

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    For most organic reaction mixture purifications the process is fairly straightforward. Use hexane/ethyl acetate or, for polar compounds, DCM/MeOH.  But what do you do if this doesn't work and your compounds are basic organic amines?

    In this post, I re-examine the options available to achieve an acceptable organic amine purification when typical separation methods are insufficient.

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    How Much Is My Flash Chromatography System Really Going to Cost?

    November 15, 2019 at 3:01 PM / by John Urh posted in Chromatography, HPLC, Media

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    This, of course, is always one of the first questions an organic, medicinal, or peptide chemist has when starting the research process for a flash chromatography system. Here at Biotage, we receive this question hundreds and hundreds of times a year, likely within the first couple of minutes of any conversation.

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    Organic Chemistry Workflow – Typical Steps and Equipment

    November 12, 2019 at 3:04 PM / by Bob Bickler posted in Chromatography, Synthesis, Workflow

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    Synthetic organic chemistry is the genesis of new pharmaceutical and commercial chemical products. In short, it is based on the idea that two or more carbon-based compounds can be forced to react using heat, or other energy source, to create a new, novel product – but this we already know.

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    What if you could use Semi-Preparative HPLC columns on your Flash Chromatography system?

    October 24, 2019 at 2:08 PM / by Panagiotis Ioannidis posted in Chromatography, HPLC

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    The newly released Biotage® Selekt flash chromatography instrument can be run at a maximum flow-rate of 300 mL/min or a maximum pressure of 30 bar. These high flowrates and pressures enable a user to perform chromatography using not only dry-packed, single-use plastic flash columns containing small (≥20 μm) spherical silica particles, but also semi-preparative, slurry-packed
    HPLC columns for multiple use with smaller (≤20 μm) spherical silica particles.

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    Biotage vs Biotage, and the winner is...

    October 23, 2019 at 9:28 AM / by Panagiotis Ioannidis posted in Chromatography, Optimization

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    When Isolera™ was launched, the maximum system pressure that could be reached was 10 bars, but reaching that pressure was a challenge since most of the Flash columns could not withstand the higher pressures. The maximum pressure rating for the Biotage® SNAP columns, for example, is limited to five or seven bars, depending on the size, and columns from most of manufacturers have the same limitation.

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    When should I use dry loading instead of liquid loading with flash column chromatography?

    October 4, 2019 at 6:17 PM / by Bob Bickler posted in Chromatography, Small Molecule, Polar, Samplet(R), Synthesis, Workflow, Media, Loading method

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    Many microwave assisted organic synthesis (MAOS) reactions use polar solvents such as alcohols, DMF, DMSO, because they absorb and transfer microwave energy very efficiently.  However, the downside of using polar, microwave absorbing solvents is that they can interfere with normal-phase flash chromatography.

    In this post, I discuss why dry loading can be advantageous when purifying polar-solvated reaction mixtures.

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    How many ways can you load sample on your column?

    May 31, 2019 at 4:02 PM / by Raffaella Bombarda posted in Chromatography, Samplet(R), Sfär, Media, Loading method

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    Biotage®, a pioneer in Flash Purification, launched the unique, removable cap SNAP flash chromatography columns in 2007. This beneficial column design feature continues with the newest Biotage flash columns named Sfär columns.

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    How do particle size and flow rate affect normal-phase flash column chromatography

    May 7, 2019 at 5:19 PM / by Bob Bickler posted in Chromatography, Media

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    Media particle size and solvent flow rate play major roles in chromatographic separations including flash purification.  This is true in both reversed-phase chromatography (aka partition chromatography) as well as normal-phase chromatography. The roles played are related to the overall compound mass-transfer kinetics and diffusion/dispersion as they migrate through the column.  Smaller particles reduce sample dilution by reducing interstitial volume, while flow rate impacts the ability of molecules to efficiently pass through the porous particles. In this post, I will show how both particle size and flow rate impact flash chromatography.

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    Greener flash chromatography tips

    April 30, 2019 at 10:18 PM / by Bob Bickler posted in Chromatography, Solvents, Media

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    In our more environmentally aware climate, chemical and pharmaceutical companies now prioritize reducing organic solvent use in chemistry labs. Employees and shareholders alike are pushing their companies to become greener which impacts how chemistry, both synthesis and purification, is performed.

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    So, what exactly is a column volume in flash column chromatography and how is it determined?

    April 10, 2019 at 6:36 PM / by Bob Bickler posted in Chromatography, volume column

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    In many of my previous posts I have used the term column volume, typically abbreviated as CV, as a value used to help determine separation quality and loading capacity.  However, I recently was asked a question about this topic from a chemist who understands the column volume concept but wanted to better understand its definition and how it is determined.

    In this post I will explain what a column volume is and how it is determined empirically.

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    Webinar: A Roadmap to Successful Chromatography

    March 19, 2019 at 10:00 PM / by Bob Bickler posted in Chromatography, Webinar

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    On July 26th, 2018, Bob Bickler, Senior Technical Specialist, recorded a webinar on A Roadmap to Successful Flash Chromatography. To learn more, read the description below as well as watch the recording!

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    The three reasons you should be using step gradients for flash column chromatography

    March 5, 2019 at 9:34 PM / by Bob Bickler posted in Chromatography, Gradient, Normal phase

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    For most synthesis and natural product chemists, flash chromatography is the primary tool for purification and isolation of compounds of interest. Purification methods include flash system defaulted linear gradients (e.g. 0-100%), active gradient modification (on-the-fly) during purification, and unique method creation based one either the chemist’s experience or TLC data (typically a linear gradient).

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    Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

    February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt, Media, Loading method

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    Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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    Are you in control of your Flash Purification?

    January 29, 2019 at 10:33 AM / by Greg Saunders posted in Chromatography

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    Learn more about how to get in control of your Flash Purification.

    With Biotage® Selekt and Sfär chemists can be in full control of Flash Purification. 
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