Welcome to the Biotage Flash Purification Blogs.

      When should I use dry loading instead of liquid loading with flash column chromatography?

      October 4, 2019 at 6:17 PM / by Bob Bickler posted in cartridge, Celite, chromatography, DCM, Developments, DMF, dry load, MAOS, microwave, polar, Samplet(R), silica, synthesis, workflow


      Many microwave assisted organic synthesis (MAOS) reactions use polar solvents such as alcohols, DMF, DMSO, because they absorb and transfer microwave energy very efficiently.  However, the downside of using polar, microwave absorbing solvents is that they can interfere with normal-phase flash chromatography.

      In this post, I discuss why dry loading can be advantageous when purifying polar-solvated reaction mixtures.

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      Which injection solvents should I use for reversed-phase flash purification?

      September 20, 2019 at 8:21 PM / by Bob Bickler posted in DMF, reversed-phase, DMSO, dissolution


      In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase flash chromatography has steadily increased over the past few years I thought it would be a good idea to discuss one of the most important factors impacting its success.

      In this post I discuss the results of some of my original research studying the impact of injection solvent choice on reversed-phase flash separations.

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