Welcome to the Biotage Flash Purification Blogs.

      How many ways can you load sample on your column?

      May 31, 2019 at 4:02 PM / by Raffaella Bombarda posted in dry load, samplet, Samplet(R), flash chromatography, Flash column chromatography, column, silica column, flash purification, sfär, loading capacity

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      Biotage®, a pioneer in Flash Purification, launched the unique, removable cap SNAP flash chromatography columns in 2007. This beneficial column design feature continues with the newest Biotage flash columns named Sfär columns.

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      Dry loading media options – diatomaceous earth

      May 28, 2019 at 10:47 PM / by Bob Bickler posted in dry load, diatomaceous earth

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      Various flash chromatography sample loading options are available including liquid and dry loading. Choosing the right technique is important because your sample loading choices (sample solvent and dry load sorbent), can have a major impact on the results.

      In this post, I compare the two techniques and show the benefits dry loading with a form of diatomaceous earth can bring to your purification.

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      Which dry load sorbent should I use for flash column chromatography?

      February 19, 2019 at 10:55 PM / by Bob Bickler posted in dry load, silica, media, sorbent

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      This is an interesting question that does not have a straightforward answer. In fact, there are many materials that are potentially useful sorbents for dry loading crude mixtures. Some of the more popular are silica, diatomaceous earth (e.g. ISOLUTE® HM-N, Celite®), alumina, and Florisil®. The sorbent choice can influence your purification results because each of the available media have different chemistry and capacity. In most cases, sample/sorbent reactivity really is not a major concern, though it can occur. What is important is the sorbent’s capacity to adsorb/absorb all of your sample and the ratio of your crude sample to the amount of dry load sorbent.

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      Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

      February 5, 2019 at 8:10 PM / by Bob Bickler posted in dry load, silica, Flash column chromatography, sfär, Selekt

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      Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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