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      Flash column chromatography equilibration speed – how fast can you go?

      April 9, 2019 at 11:22 PM / by Bob Bickler posted in equilibration, speed, media


      Equilibrating silica flash chromatography columns is something I always do.  There are chemists who see this as an unnecessary, time-and-solvent-wasting step.  However, because getting consistent, predictable results is a priority for me, I equilibrate to remove the variability that can be caused by heat generated as solvent initially contacts the silica. Consistency is really important when running flash column chromatography because re-runs are time consuming and may put your compound at risk.

      In this post, I examine the role of equilibration speed and duration to show its impact, or lack there of, on purification performance.

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