Welcome to the Biotage Flash Purification Blogs.

    How does an acidic pH affect reversed-phase chromatography separations?

    November 1, 2022 at 2:19 PM / by Bob Bickler posted in Reversed-phase, flash chromatography, separation

    0 Comments

    Chromatography is as much an art as it is a science. Between synthetic reaction products and natural products, the range of compounds requiring separation, purification, and isolation is broad and diverse creating challenges from time to time. Because of this diversity, not all chromatographic separations can be performed with a “neutral” solvent system – one without added pH modifiers or buffers.

    Read More

    How to avoid sample blowback when liquid loading

    May 24, 2022 at 2:19 PM / by Bob Bickler posted in flash chromatography, sample, liquid, pressure

    0 Comments

    Ever experience the problem where you load your dissolved sample into a flash column via a syringe only to encounter either resistance or “blowback”? Blowback is a term describing the situation where your sample does not stay in the column but flows back out of the column’s cap after the loading syringe is removed.

    Read More

    Do It Yourself: Flash Chromatography Test Mix

    May 12, 2022 at 7:19 PM / by Charles Parsons posted in flash chromatography, DIY, parabens

    1 Comment

    In today’s post I’d like to discuss how you can check your Biotage flash system to ensure it is running smoothly and still performing at the high level and capability that is expected of all Biotage products. I’ll go over how to set up and run the Sfär-TM1 parabens test, what to look for as indicators of the “health” of the system, and what you can do if anything looks amiss.

    Read More

    How many times can I reuse my reversed-phase flash column?

    February 22, 2022 at 2:41 PM / by Bob Bickler posted in Reversed-phase, Reuse, flash chromatography

    2 Comments

    Reversed-phase flash chromatography use continues to increase for a variety of reasons. Unlike silica normal-phase flash columns, which typically are used only once, reversed-phase flash columns can be cleaned, stored, and reused. How many times can a column be reused is a frequent question I receive. In this post, I will do my best to answer this question.

    Read More

    Can Reaction Temperature Impact Synthetic Product Yield and Purity?

    November 17, 2021 at 7:01 PM / by Bob Bickler posted in reaction, flash chromatography

    0 Comments

    Though this is a purification blog I do, from time to time, like to address synthetic chemistry experimentation findings in the desire to assist you with your reactions, as this is the front-end of your synthesis workflow. So, in this post, I report on some findings of the effect of reaction temperature on the synthesis of an amide, 2-amino-N-benzylbenzamide, a potential antibacterial compound[1].

    Read More

    Can dry load media choice impact normal-phase flash purification quality?

    June 2, 2021 at 4:28 PM / by Bob Bickler posted in Normal Phase, dry load, flash chromatography

    0 Comments

    Flash chromatography is a purification technique used by chemists to isolate their targeted compound from by-products and impurities. Because the reaction mixture (or natural product extract) may have its best solubility in a solvent that is chromatographically “stronger” than the mobile phase, liquid sample loading can be problematic causing early-eluting, broad peaks which can reduce purification efficacy and product purity. In those cases, a technique called dry loading is a better alternative.

    Read More