As the popularity of prep-scale, reversed-phase flash chromatography increases, so does the frequency that I get asked this question, "How do I determine loading capacity in reversed-phase flash chromatography?"
In the world of HPLC, loading capacity isn’t normally a concern as it is primarily an analytical technique. In the synthetic organic chemistry world, most purification is performed with silica gel where flash column purification methods are developed and loading capacity estimated from TLC data. However, when normal-phase flash does not work and reversed-phase flash is needed, the question of how to determine reversed-phase loading capacity comes up.
In this post I will attempt to provide some guidelines to help you understand and determine reversed-phase flash chromatography loading capacity.