Welcome to the Biotage Flash Purification Blogs.

    How does a flash system’s internal volume affect gradients?

    November 8, 2022 at 9:21 PM / by Bob Bickler posted in gradient, volume

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    For many years I have been publishing a blog on flash chromatography, especially related to its theory and application. It dawned on me today that one area I have not yet addressed is the impact of the flash system’s internal plumbing volume on a programmed gradient, so, here it is.

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    What is a step gradient and why should I use it?

    September 27, 2022 at 2:07 PM / by Bob Bickler posted in gradient, step gradient

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    Most chemists I meet utilizing flash chromatography use linear gradients. These gradients are either very generic (X% to Y% over a certain time or volume) or can be complex with multiple slope changes and even isocratic holds. These complex gradients are often created during a purification to get the desired separation because the generic gradient does not provide enough separation for the target compound needing purification.

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    How should normal-phase gradient solvents be chosen?

    March 14, 2022 at 1:43 PM / by Bob Bickler posted in Solvents, Normal Phase, gradient

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    Normal-phase flash chromatography is an integral component of the organic synthesis workflow. Since reactions rarely generate 100% pure product, they need purification and flash chromatography is the most utilized tool for that task.

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