Welcome to the Biotage Flash Purification Blogs.

    How can I reduce flash column purification time and cost?

    May 8, 2019 at 2:57 PM / by Bob Bickler posted in Chromatography Fundamentals, Reversed-phase, Solvents, Troubleshooting and Optimization, Green, Sfär, Selekt, Media and Resin, Cost, Normal Phase

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    This is a question being asked of my colleagues and me more and more frequently, especially in pharma accounts.  Why?  Well, you are familiar with the adage – Time is Money, right.  Well this really applies to them. A new molecular entity (NME) created as a pharmaceutical can take up to a decade and a billion dollars to bring to market.  Granted, the biggest costs are in the clinical trials but the synthetic route and the time to discover and make the compound – and purify it – plays a major role within drug discovery and development. This timeline is not helped by the ever increasingly difficult-to-synthesize compounds being investigated as drug candidates today.

    With that in mind, this post focuses on ways to speed the purification process without sacrificing purity and yield.

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    Greener flash chromatography tips

    April 30, 2019 at 10:18 PM / by Bob Bickler posted in Green, Sfär, Cost, Normal Phase

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    In our more environmentally aware climate, chemical and pharmaceutical companies now prioritize reducing organic solvent use in chemistry labs. Employees and shareholders alike are pushing their companies to become greener which impacts how chemistry, both synthesis and purification, is performed.

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    How to Choose Between Linear Gradients and Step Gradients for Flash Chromatography

    April 12, 2019 at 12:51 PM / by Bob Bickler posted in Chromatography Fundamentals, Green, Media and Resin

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    Varying the concentrations of mobile phase solvents during  flash purification chromatography enhances the ability of the technique to effectively isolate the desired compound from reaction by-products and unconsumed reagents.  Choosing how these concentrations will be varied over time has a significant effect on the purity and recovery of desired compounds.

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    Why is reversed-phase flash chromatography use increasing?

    April 3, 2019 at 4:18 AM / by Bob Bickler posted in Reversed-phase, Green, Sfär, Selekt, Cost

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    Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth despite the fact that reversed-phase flash columns are considerably more expensive than silica columns and you need to evaporate water from your fractions. So, what’s driving this change in chemists’ modus operandi?

    In this post, I will explain why chemists are increasingly using reversed-phase flash chromatography for routine, intermediate, and final compound purification and provide and example as well.

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    Are so-called “high performance” flash columns worth their higher prices?

    March 19, 2019 at 10:05 PM / by Bob Bickler posted in Reversed-phase, Green, Sfär, Cost, Normal Phase

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    Most flash column manufacturers now offer “high performance” flash chromatography columns with the promise of higher loading, increased purity, and even reduced solvent consumption. Working for Biotage, I have made those valid claims for our products as well.

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    The three reasons you should be using step gradients for flash column chromatography

    March 5, 2019 at 9:34 PM / by Bob Bickler posted in Chromatography Fundamentals, Solvents, Green, Sfär, Selekt, Scale-Up, Cost, Normal Phase

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    For most synthesis and natural product chemists, flash chromatography is the primary tool for purification and isolation of compounds of interest. Purification methods include flash system defaulted linear gradients (e.g. 0-100%), active gradient modification (on-the-fly) during purification, and unique method creation based one either the chemist’s experience or TLC data (typically a linear gradient).

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    Are you in control of your Flash Purification?

    January 29, 2019 at 10:33 AM / by Greg Saunders posted in Green, Sfär, Selekt, Cost

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    Learn more about how to get in control of your Flash Purification.

    With Biotage® Selekt and Sfär chemists can be in full control of Flash Purification. 
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    Webinar: Inspiring Productivity with Modern Flash Chromatography

    December 11, 2018 at 7:40 PM / by Bob Bickler posted in Chromatography Fundamentals, Reversed-phase, Green, Sfär, Selekt, Webinar

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    On December 6th, 2018, Bob Bickler, Senior Technical Specialist, recorded a webinar on Inspiring Productivity with Modern Flash Chromatography. To learn more, read the description below as well as watch the recording!

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    Building greener separations into the flash workflow

    November 20, 2018 at 3:01 PM / by Greg Saunders posted in Chromatography Fundamentals, Workflow, Green, Sfär, Selekt

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    The synthesis of new chemical entities certainly has a high environmental cost due to the chemicals employed, especially in flash column chromatography, where large quantities of solvents are routinely used. The previous article in this series presented three strategies that can greatly reduce the environmental impact of your flash purifications: (1) purifying more product with less silica, and solvent, (2) making green chemistry work for you, such as changing to more environmentally friendly solvents, and (3) optimizing chromatography with more effective step gradients. Let’s look at how new developments in column design, simplifications in step gradients, and support from a leader in flash column chromatography can help in making your operation greener.

     

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    Biotage® Sfär

    October 1, 2018 at 1:17 PM / by Raffaella Bombarda posted in Chromatography Fundamentals, Green, Sfär, Media and Resin, Scale-Up

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    Sfär Stands for Spherical, Biotage® Stands for Quality 

    Biotage developed and introduced pre-packed columns for flash purification in 1994. Today our broad selection of columns enables professionals to choose the solution which best suits their purification needs.

    Sfär is the Swedish word for "sphere", and the name of our columns reflects the fact that we have made spherical silica a standard due to its higher surface area and higher loading capacity. Reliable and flexible, Biotage® Sfär columns deliver larger loading capacities, tighter elution bands and purer fractions than traditional flash columns.
    Biotage® Sfär is a complete portfolio of flash columns, available in a large variety of sizes from 5g to 350 g and in a range of media types, so you can purify milligrams to multi-grams of your valuable compounds quickly and easily.

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    How tools such as flash column chromatography boost confidence in drug discovery

    September 26, 2018 at 3:54 PM / by Kathryn Lawrence posted in Workflow, Green, Sfär, Selekt, Automation, Cost

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    New drug substances, the result of more than a decade of pharmaceutical research and development,  have revolutionized society by saving lives, increasing life-spans, reducing suffering, avoiding surgery, and shortening hospital stays. New breakthroughs in the treatment of non-communicable diseases mean that drug substances are in a constant state of high demand, making the pharmaceutical industry one of the most innovative of industries on the globe. Why then, do so few drugs come to market? It turns out that a key factor is providing drug discovery chemists with tools such as automated flash chromatography to ensure that they can be confident in delivering target compounds in a timely fashion.

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    How to work smarter with flash column chromatography

    September 5, 2018 at 4:54 PM / by Bob Bickler posted in Workflow, Green, Sfär, Selekt, Webinar, Cost

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    First, join me on a flashback to my past as a discovery chemist just fresh out of grad school and eager to make a difference in pharmaceutical research. I was advised by my boss to model my behavior after a colleague and labmate with a reputation of being highly productive and successful. I was also informed that ‘chemist productivity’ was measured by 1) the number of compounds (of sufficient quantity and quality) he/she registered in the company’s database and 2) meeting project milestones.

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    Reducing Environmental Impact of Chemical Purification in Drug Discovery

    August 28, 2018 at 7:44 PM / by Greg Saunders posted in Chromatography Fundamentals, Green, Sfär, Selekt, Cost

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    Chemistry, by its very nature, involves the use of chemicals that can be harmful, toxic and potentially damaging to the environment, which means that drug discovery currently has a large and expensive environmental footprint. However, all is not lost. With a few small steps it is possible to make a big change to the impact that drug discovery has on our world. Let’s look at ways we can reduce the environmental impact of chemical processing and flash chromatography. This involves using green chemistry applications in the purification workflow to find a solution that reduces chemical waste to make it good for both the chemist and the environment.

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    Three Key Strategies that make Flash Purification Greener

    August 28, 2018 at 7:34 PM / by Greg Saunders posted in Green, Sfär, Selekt, Cost

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    Chromatographic purification methods such as flash chromatography can have a high environmental impact since they typically involve large quantities of harmful or toxic solvents run at high flow rates. In many laboratories the cost of procuring and then disposing of these solvents can be a major part of the overall cost of projects. For the chemist, trying to reduce the environmental impact of small molecule synthesis is part of being a good global citizen. Here are three strategies that greatly reduce the environmental impact of flash purifications. It can also save money in the long run by reducing the cost of chemical waste disposal.

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