Welcome to the Biotage Flash Purification Blogs.

      What is Flash Chromatography and why should I do it?

      August 20, 2019 at 5:34 PM / by Bob Bickler posted in side reactions, purify, impurity

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      Flash chromatography is a chemical separation technique used to purify chemical mixtures. Because it is a purification technology, the process is also referred to as flash purification.

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      How changing stationary phase chemistry can impact separation selectivity

      June 12, 2019 at 2:42 AM / by Bob Bickler posted in selectivity, stationary phase, impurity

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      Challenging separations, we all have faced this vexing problem. You synthesized your compound, analyzed it, and know your molecule is in there, based on LC-MS or TLC. Then, you do some method development using a silica TLC plate and see a major spot with some minor, early-eluting impurities. You think that the purification will be easy only to find that your “purified” compound has some co-eluting impurities. Now what? Should you change solvents or change stationary phase?

      In this post, I will show how changing the column media but keeping the same solvents removed a co-eluting impurity in one of my reaction mixes.

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