Welcome to the Biotage Flash Purification Blogs.

    Which loading method should I use for purification by flash chromatography?

    July 10, 2019 at 3:14 PM / by Bob Bickler posted in Loading Techniques, Normal Phase, Isolera

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    Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables.  In previous posts I have talked about selecting the best solvents, their ratios, and maximizing load based on TLC Rf data. These are all important chromatography-generated variables but now I would like to share some tips on actual technique differences and their impact on purification performance.

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    Increasing Productivity with Automated Flash Purification - Rice University Customer case

    April 16, 2019 at 10:59 PM / by Sarah Moran posted in Synthesis, Sfär, Customer case, Isolera

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    "50% of my time as a Ph.D. was spend baby-sitting columns," stated Dr. László Kürti during our interview in February. Keep reading to find out more about how his team was able to increase their productivity by implementing Automated Flash Purification into their lab work.

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    Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

    February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography Fundamentals, Sfär, Selekt, Media and Resin, Loading Techniques, Isolera

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    Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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    The value of in-line mass detection with flash column chromatography

    December 11, 2018 at 7:56 PM / by Bob Bickler posted in Reversed-phase, Sfär, Detector, Normal Phase, Isolera

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    For many chemists, flash chromatography with UV-triggered fractionation is part of their everyday workflow. Prior to flash chromatography, the reaction mixtures are either analyzed by TLC, analyzed by LC-MS, or both to ensure the targeted product has been synthesized. But, what if the reaction created a lot of by-products? How do you find your product in a sea of impurities? In this post, I will discuss how using a flash purification system with an in-line mass detector will simplify flash purification and isolate the target molecule or molecules. 

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    This is how you can use Biotage Sfär columns on your Isolera

    December 6, 2018 at 10:09 AM / by Raffaella Bombarda posted in Sfär, Isolera

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    From October 1st, 2018 Biotage® Sfär columns are available and they appear automatically on the Selekt instrument. Learn more about Selekt and Sfär.

    If you run your Flash purification on Isolera® and you would like to start testing the new Sfär columns, there is an easy way of doing it. You can look at Bob doing it in the lab or just download and follow the instructions below.

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