Welcome to the Biotage Flash Purification Blogs.

    How should I make my linear gradient?

    October 11, 2022 at 2:20 PM / by Bob Bickler posted in linear gradient, resolution

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    Flash chromatography gradients need to provide as good of a separation between the target compound at its closest eluting by-products as possible in order to maximize product purity and load. Many chemists create methods based on a history with a compound class, knowing what has worked in the past. If no history with a synthetic compound class exists, then methods need developing.

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    When is Gradient Elution Better than Isocratic Elution?

    March 30, 2022 at 2:00 PM / by Bob Bickler posted in isocratic, linear gradient, step gradient


    Gradient or isocratic elution, that is the question. Within flash chromatography these are the options afforded to separate reaction mixtures and natural product extracts and is the focus of this post.

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