Welcome to the Biotage Flash Purification Blogs.

      How important is your flash column’s plate count, aka efficiency, to your purification?

      October 29, 2019 at 5:00 PM / by Bob Bickler posted in efficiency, loading capacity, plate count

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      Plate count is a theoretical number describing the separation efficiency of a chromatography column. In short, it is a measure an eluting compound's bandwidth at the time it elutes from a column, Equation 1.

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      How many ways can you load sample on your column?

      May 31, 2019 at 4:02 PM / by Raffaella Bombarda posted in dry load, samplet, Samplet(R), flash chromatography, Flash column chromatography, column, silica column, flash purification, sfär, loading capacity

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      Biotage®, a pioneer in Flash Purification, launched the unique, removable cap SNAP flash chromatography columns in 2007. This beneficial column design feature continues with the newest Biotage flash columns named Sfär columns.

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      Can reversed-phase flash chromatography purify better than normal-phase?

      May 9, 2019 at 4:44 PM / by Bob Bickler posted in normal phase, reversed-phase, loading capacity

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      The answer to this question is yes, reversed-phase can sometimes provide a better separation and thus better purification than normal-phase.  When is reversed-phase likely to be the better choice is a different, and likely better, question.

      In this post I will try to demonstrate when reversed-phase is likely the better purification mode.

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      How do particle size and flow rate affect normal-phase flash column chromatography

      May 7, 2019 at 5:19 PM / by Bob Bickler posted in flash column, Flash column chromatography, loading capacity

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      Media particle size and solvent flow rate play major roles in chromatographic separations including flash purification.  This is true in both reversed-phase chromatography (aka partition chromatography) as well as normal-phase chromatography. The roles played are related to the overall compound mass-transfer kinetics and diffusion/dispersion as they migrate through the column.  Smaller particles reduce sample dilution by reducing interstitial volume, while flow rate impacts the ability of molecules to efficiently pass through the porous particles. In this post, I will show how both particle size and flow rate impact flash chromatography.

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