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    What is the maximum flash column sample load volume?

    April 12, 2022 at 9:40 PM / by Bob Bickler posted in loading capacity, solvent

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    A fairly common question I receive is "how much of my reaction mixture can I load on my flash chromatography column?". To this question there is not a cut and dry answer because of a number of variables which influence the outcome. To help illustrate this, I will show in this post the results of a study I performed in my lab with one of my reaction mixtures.

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    What is the maximum volume I can load on my reversed-phase flash column?

    May 19, 2021 at 2:11 PM / by Bob Bickler posted in Reversed-phase, Solvents, loading capacity

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    With all forms of chromatography there are limitations relating to sample load – both mass and volume. These are independent variables which, for the best results, should be investigated separately. In this post, I will address the impact of increasing solvent volume on flash chromatographic separations.

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    What is my C18 flash column's loading capacity?

    May 5, 2021 at 2:27 PM / by Bob Bickler posted in Reversed-phase, loading capacity

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    A good question I get asked frequently to which there is no specific value. When asked this question I answer, “it depends on your sample and how good your separation is”; not a satisfying response, but it is the truth.

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