In past posts I discussed which solvents work best for sample loading in reversed-phase flash chromatography. Recently, I was asked to provide some insight as which solvents are best in normal-phase flash column chromatography.
Liquid loading of samples onto chromatography columns is not always straight-forward. If your sample is dissolved in the mobile phase, that can work but may not be the best choice, especially if running a gradient.
In this post I show you some work I have done that opens up quite a few options for solvents used for loading samples and some surprising results with DMF.