Welcome to the Biotage Flash Purification Blogs.

    Which dry load sorbent should I use for flash column chromatography?

    February 19, 2019 at 10:55 PM / by Bob Bickler posted in Sfär, Selekt, Media and Resin, Loading Techniques

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    This is an interesting question that does not have a straightforward answer. In fact, there are many materials that are potentially useful sorbents for dry loading crude mixtures. Some of the more popular are silica, diatomaceous earth (e.g. ISOLUTE® HM-N, Celite®), alumina, and Florisil®. The sorbent choice can influence your purification results because each of the available media have different chemistry and capacity. In most cases, sample/sorbent reactivity really is not a major concern, though it can occur. What is important is the sorbent’s capacity to adsorb/absorb all of your sample and the ratio of your crude sample to the amount of dry load sorbent.

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    Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

    February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography Fundamentals, Sfär, Selekt, Media and Resin, Loading Techniques, Isolera

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    Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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    Dry loading vs. liquid loading, which provides better flash column chromatography results?

    January 23, 2019 at 2:15 PM / by Bob Bickler posted in Chromatography Fundamentals, Media and Resin, Loading Techniques

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    When it comes time to purify your reaction mixture or natural product extract, you have a choice to make. Should you simply load your dissolved sample onto your flash column or take the extra step to adsorb your mix onto a sorbent and dry it before loading? The choice can have a major impact on your results.

    In this post, I will share results of work I conducted in my lab, comparing liquid and dry loading a reaction mixture that containing eight major components.

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