Welcome to the Biotage Flash Purification Blogs.

    What do I do if a 2-solvent gradient will not separate my sample?

    February 11, 2020 at 3:12 PM / by Bob Bickler posted in Gradient, Normal phase, Optimization, Solvents, Mass-directed purification

    0 Comments

    Usually, a 2-solvent or binary gradient will separate your desired compound from the by-products and impurities. Sometimes though, you can encounter a mixture in which some compounds co-elute and are not separable with any binary gradient you try.

    I encountered this situation recently while trying to purify a lavender essential oil and have dedicated this post to how I solved the problem. 

    Read More

    Why is reversed-phase flash chromatography use increasing?

    April 3, 2019 at 4:18 AM / by Bob Bickler posted in Normal phase, Optimization, Reversed-phase, Mass-directed purification

    0 Comments

    Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth despite the fact that reversed-phase flash columns are considerably more expensive than silica columns and you need to evaporate water from your fractions. So, what’s driving this change in chemists’ modus operandi?

    In this post, I will explain why chemists are increasingly using reversed-phase flash chromatography for routine, intermediate, and final compound purification and provide and example as well.

    Read More