Welcome to the Biotage Flash Purification Blogs.

      Flash column chromatography equilibration speed – how fast can you go?

      April 9, 2019 at 11:22 PM / by Bob Bickler posted in equilibration, heat, speed, media

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      Equilibrating silica flash chromatography columns is something I always do.  There are chemists who see this as an unnecessary, time-and-solvent-wasting step.  However, because getting consistent, predictable results is a priority for me, I equilibrate to remove the variability that can be caused by heat generated as solvent initially contacts the silica. Consistency is really important when running flash column chromatography because re-runs are time consuming and may put your compound at risk.

      In this post, I examine the role of equilibration speed and duration to show its impact, or lack there of, on purification performance.

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      Which dry load sorbent should I use for flash column chromatography?

      February 19, 2019 at 10:55 PM / by Bob Bickler posted in dry load, silica, media, sorbent

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      This is an interesting question that does not have a straightforward answer. In fact, there are many materials that are potentially useful sorbents for dry loading crude mixtures. Some of the more popular are silica, diatomaceous earth (e.g. ISOLUTE® HM-N, Celite®), alumina, and Florisil®. The sorbent choice can influence your purification results because each of the available media have different chemistry and capacity. In most cases, sample/sorbent reactivity really is not a major concern, though it can occur. What is important is the sorbent’s capacity to adsorb/absorb all of your sample and the ratio of your crude sample to the amount of dry load sorbent.

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