Welcome to the Biotage Flash Purification Blogs.

      How can I perform normal-phase and reversed-phase column chromatography on one flash system?

      May 20, 2019 at 2:21 PM / by Bob Bickler posted in normal phase, reversed-phase

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      For chemists, flash chromatography is part of their everyday synthesis workflow. For most syntheses, crude reaction mixtures are purified by normal-phase (aka adsorption) chromatography.  There are times, however, where the crude mixture’s complexity and polarity make normal-phase chromatography very challenging.  For these situations, reversed-phase (aka partition) chromatography may be a preferred option.

      But, if you have only one flash system available, can you, should you, and how do you efficiently switch from non-polar, normal-phase solvents to polar, reversed-phase solvents – and back again without issues? In this post I'll attempt to shed some light on the topic.

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      Can reversed-phase flash chromatography purify better than normal-phase?

      May 9, 2019 at 4:44 PM / by Bob Bickler posted in normal phase, reversed-phase, loading capacity

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      The answer to this question is yes, reversed-phase can sometimes provide a better separation and thus better purification than normal-phase.  When is reversed-phase likely to be the better choice is a different, and likely better, question.

      In this post I will try to demonstrate when reversed-phase is likely the better purification mode.

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      Why is reversed-phase flash chromatography use increasing?

      April 3, 2019 at 4:18 AM / by Bob Bickler posted in co-elution, Mass Detection, normal phase, reversed-phase

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      Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth despite the fact that reversed-phase flash columns are considerably more expensive than silica columns and you need to evaporate water from your fractions. So, what’s driving this change in chemists’ modus operandi?

      In this post, I will explain why chemists are increasingly using reversed-phase flash chromatography for routine, intermediate, and final compound purification and provide and example as well.

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      The three reasons you should be using step gradients for flash column chromatography

      March 5, 2019 at 9:34 PM / by Bob Bickler posted in linear gradient, normal phase, step-gradient, greener flash purification

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      For most synthesis and natural product chemists, flash chromatography is the primary tool for purification and isolation of compounds of interest. Purification methods include flash system defaulted linear gradients (e.g. 0-100%), active gradient modification (on-the-fly) during purification, and unique method creation based one either the chemist’s experience or TLC data (typically a linear gradient). Rarely do chemists work to optimize the purification to maximize target compound purity by employing a step gradient. In this post, I discuss the value optimized step gradients provide chemists.

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