Welcome to the Biotage Flash Purification Blogs.

    Determining solvent strength in flash column chromatography

    May 7, 2019 at 5:23 PM / by Bob Bickler posted in Optimization, Solvents

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    Recently, one of our readers wrote and asked how to determine solvent strength in normal-phase flash chromatography. This is an excellent question because solvent strength is one of several factors impacting flash chromatography performance.

    In this post I will explain how solvent strength can easily be determined.

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    How to Choose Between Linear Gradients and Step Gradients for Flash Chromatography

    April 12, 2019 at 12:51 PM / by Bob Bickler posted in Gradient, Optimization

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    Varying the concentrations of mobile phase solvents during  flash purification chromatography enhances the ability of the technique to effectively isolate the desired compound from reaction by-products and unconsumed reagents.  Choosing how these concentrations will be varied over time has a significant effect on the purity and recovery of desired compounds.

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    Flash column chromatography equilibration speed – how fast can you go?

    April 9, 2019 at 11:22 PM / by Bob Bickler posted in Optimization, Media

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    Equilibrating silica flash chromatography columns is something I always do.  There are chemists who see this as an unnecessary, time-and-solvent-wasting step.  However, because getting consistent, predictable results is a priority for me, I equilibrate to remove the variability that can be caused by heat generated as solvent initially contacts the silica. Consistency is really important when running flash column chromatography because re-runs are time consuming and may put your compound at risk.

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    Why is reversed-phase flash chromatography use increasing?

    April 3, 2019 at 4:18 AM / by Bob Bickler posted in Normal phase, Optimization, Reversed-phase, Mass-directed purification

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    Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth despite the fact that reversed-phase flash columns are considerably more expensive than silica columns and you need to evaporate water from your fractions. So, what’s driving this change in chemists’ modus operandi?

    In this post, I will explain why chemists are increasingly using reversed-phase flash chromatography for routine, intermediate, and final compound purification and provide and example as well.

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    Are so-called “high performance” flash columns worth their higher prices?

    March 19, 2019 at 10:05 PM / by Bob Bickler posted in Optimization, Media

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    Most flash column manufacturers now offer “high performance” flash chromatography columns with the promise of higher loading, increased purity, and even reduced solvent consumption. Working for Biotage, I have made those valid claims for our products as well.

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    Are there benefits to collecting flash chromatography fractions based on multiple UV wavelengths?

    January 8, 2019 at 7:29 PM / by Bob Bickler posted in Optimization, Selekt

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    UV detection and fractionation is ubiquitous in flash chromatography. It is the default methodology used to detect and collect eluting compounds. Today’s flash chromatography systems offer UV-triggered fractionation on one, two, or a range of wavelengths in order to either increase fractionation specificity, yield, or increase sensitivity.

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