Welcome to the Biotage Flash Purification Blogs.

    How to isolate impurities from a reaction product

    June 7, 2022 at 5:57 PM / by Bob Bickler posted in reaction, orthogonal, by-product, impuriity

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    Ensuring that the product of a reaction is pure is critical to that compound’s viability as a marketable entity. Impurities often require characterization if they are in excess of regulated limits. The isolation of these compounds can be quite challenging. 

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    Can maximizing product yield and purity from messy reaction mixture be green?

    October 5, 2021 at 8:42 PM / by Bob Bickler posted in Green, purification, reaction, orthogonal

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    Chemical reactions gone wrong, I’m sure we all have experienced this issue, I know I have. You add your reagents in the proper amounts with a suitable solvent and perform your reaction only to find your by-product yield was greater than your product; by a lot. So, what do you do to isolate what little product you created with maximum yield and purity without breaking the proverbial bank on a big flash column and the solvent required for the purification?

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    What is Orthogonal Flash Chromatography and Why Should I do it?

    September 7, 2021 at 3:15 PM / by Bob Bickler posted in Reversed-phase, Normal Phase, orthogonal

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    Think of orthogonal flash chromatography as 2D-chromatography where a reaction mixture or natural product extract is purified first with one methodology or solvent gradient then re-purified with a different method or solvent pair in order to remove co-eluting impurities. This is a technique practiced in medicinal chemistry, especially for final compound purification, when the final product is purified first with normal-phase flash followed by reversed-phase prep HPLC.


    There are two general flash chromatography techniques...

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