Welcome to the Biotage Flash Purification Blogs.

    Does reagent and reaction solvent order impact product yield and purity?

    October 19, 2021 at 6:00 PM / by Bob Bickler posted in Solvents, Synthesis, reaction, reagent


    This is a question I asked myself while I have been studying synthesis variables to see what, if any, impact each variable has on reaction product yield and purity. For this post, I evaluated the order in which I added reactants and solvent.

    Read More

    Can maximizing product yield and purity from messy reaction mixture be green?

    October 5, 2021 at 8:42 PM / by Bob Bickler posted in Green, purification, reaction, orthogonal

    1 Comment

    Chemical reactions gone wrong, I’m sure we all have experienced this issue, I know I have. You add your reagents in the proper amounts with a suitable solvent and perform your reaction only to find your by-product yield was greater than your product; by a lot. So, what do you do to isolate what little product you created with maximum yield and purity without breaking the proverbial bank on a big flash column and the solvent required for the purification?

    Read More

    In-line Scavenging Simplifies Flash Column Chromatography Reaction Mixture Purification

    November 20, 2020 at 2:55 PM / by Bob Bickler posted in Scavenger, reaction


    I am always grateful for the feedback I get from my blog readers.  Today's blog is in response for multiple requests for tips on purifying complex mixtures and suggestions for alternative sample loading techniques.

    In this post, I will attempt to address both, to some degree anyway, with a single example using a scavenger resin.

    Read More