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    How should I make my linear gradient?

    October 11, 2022 at 2:20 PM / by Bob Bickler posted in linear gradient, resolution

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    Flash chromatography gradients need to provide as good of a separation between the target compound at its closest eluting by-products as possible in order to maximize product purity and load. Many chemists create methods based on a history with a compound class, knowing what has worked in the past. If no history with a synthetic compound class exists, then methods need developing.

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