Welcome to the Biotage Flash Purification Blogs.

      Purifying ionic compounds by flash column chromatography

      October 18, 2019 at 10:00 PM / by Bob Bickler posted in Polar, Reversed-phase, Solvents

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      One of the more challenging purifications is that of water-soluble, ionizable compounds. Typically, normal-phase with silica is not used because of the probable non-reversible interactions, especially between the ionized amines interacting and the ionizable silanols.  With normal-phase out of the purification solution that leaves ion exchange and reversed-phase as chromatographic options.

      In this post I will discuss the use of reversed-phase and the influence pH and buffers have on the chromatography of some ionic, water soluble compounds.

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      Prep HPLC vs. reversed-phase flash chromatography: How to choose?

      October 15, 2019 at 5:02 PM / by Bob Bickler posted in HPLC, Normal phase, Reversed-phase

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      This question is one that is increasing in frequency. Over the past 10 or so years reversed-phase flash chromatography use has increased dramatically. Likewise, reversed-phase preparative HPLC (RP pHPLC) use has also increased. Chemists need to know when to choose between the speed and low solvent use of flash column chromatography and the ultimate purification of RP pHPLC. With this as the backdrop, let me give you my thoughts on how to choose between flash chromatography and when it is best to use RP pHPLC.

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      How do I choose between Normal- or Reversed-phase flash column chromatography for my compound purification?

      October 4, 2019 at 6:25 PM / by Bob Bickler posted in Normal phase, Reversed-phase, Solvents

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      How to choose between normal- and reversed-phase flash column chromatography is an excellent question and one that my readers often ask.  Those who use column chromatography know that as long as the reaction products or compounds are fairly non-polar and near neutral pH they will have successful purifications.  However, when your mixture's chemical characteristics are more challenging (polar, non-polar, basic, acidic) there are other options that are available to successfully separate pure compounds.

      In this post, I will discuss the criteria you can use to guide your choice between normal- or reversed-phase flash chromatography.

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      Which injection solvents should I use for reversed-phase flash purification?

      September 20, 2019 at 8:21 PM / by Bob Bickler posted in Reversed-phase, Solvents

      6 Comments

      In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase flash chromatography has steadily increased over the past few years I thought it would be a good idea to discuss one of the most important factors impacting its success.

      In this post I discuss the results of some of my original research studying the impact of injection solvent choice on reversed-phase flash separations.

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      How can I rapidly remediate THC from CBD in my hemp extract using flash column chromatography?

      September 20, 2019 at 2:52 PM / by Bob Bickler posted in Cannabis, Gradient, Remediation, Reversed-phase, Troubleshooting

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      Tetrahydrocannabinol, aka THC, is a psychoactive compound found in cannabis and, to a lesser degree, in hemp.  Though THC is legal in some locations in the US and Canada, there is a growing market for its non-hallucinogenic cousin, cannabidiol (CBD), which has purported medical benefits.

      The problem with isolating CBD from cannabis and hemp is contamination from THC, which is typically present at a moderate to high percentage. In this post, I will provide some insight into rapidly purifying CBD to remove THC.

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      What are good reversed-phase dry load options?

      July 23, 2019 at 2:40 PM / by Bob Bickler posted in Reversed-phase, Sfär, Loading method

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      Using a “dry” loading technique with flash chromatography typically improves compound purity and overall separation quality compared to liquid loading. The reasons for this I have prophesized previously and include:

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      Pesticide remediation of Cannabis oil - Myclobutanil removal by flash chromatography

      June 11, 2019 at 3:51 PM / by Brian Wagner posted in Cannabis, Chromatography, Reversed-phase

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      Welcome to a second installment of the Biotage Cannabis Application development flash blog.  The first post, dated August 20  2016, outlined an orthogonal approach to isolating cannabinoids from winterized extract.  Give it read if you have not seen it

      Today we investigate the “hot” topic of pesticide elimination from extract.  Specifically – Myclobutanil remediation.

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      How can I perform normal-phase and reversed-phase column chromatography on one flash system?

      May 20, 2019 at 2:21 PM / by Bob Bickler posted in Normal phase, Reversed-phase

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      For chemists, flash chromatography is part of their everyday synthesis workflow. For most syntheses, crude reaction mixtures are purified by normal-phase (aka adsorption) chromatography.  There are times, however, where the crude mixture’s complexity and polarity make normal-phase chromatography very challenging.  For these situations, reversed-phase (aka partition) chromatography may be a preferred option.

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      Minor Cannabinoid Separation by Reversed-phase Flash Chromatography

      May 17, 2019 at 8:30 PM / by Bob Bickler posted in Cannabis, Chromatography, Optimization, Reversed-phase

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      Previously, I have posted on a normal-phase flash chromatography method to separate and isolate CBG from a CBD-rich hemp distillate. CBG is just one of many naturally occurring minor cannabinoids of interest in this fast-growing market.

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      Can reversed-phase flash chromatography purify better than normal-phase?

      May 9, 2019 at 4:44 PM / by Bob Bickler posted in Normal phase, Reversed-phase, Media

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      The answer to this question is yes, reversed-phase can sometimes provide a better separation and thus better purification than normal-phase.  When is reversed-phase likely to be the better choice is a different, and likely better, question.

      In this post I will try to demonstrate when reversed-phase is likely the better purification mode.

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      Why is reversed-phase flash chromatography use increasing?

      April 3, 2019 at 4:18 AM / by Bob Bickler posted in Normal phase, Optimization, Reversed-phase, Mass-directed purification

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      Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth despite the fact that reversed-phase flash columns are considerably more expensive than silica columns and you need to evaporate water from your fractions. So, what’s driving this change in chemists’ modus operandi?

      In this post, I will explain why chemists are increasingly using reversed-phase flash chromatography for routine, intermediate, and final compound purification and provide and example as well.

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