Welcome to the Biotage Flash Purification Blogs.

      How changing stationary phase chemistry can impact separation selectivity

      June 12, 2019 at 2:42 AM / by Bob Bickler posted in selectivity, stationary phase, impurity

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      Challenging separations, we all have faced this vexing problem. You synthesized your compound, analyzed it, and know your molecule is in there, based on LC-MS or TLC. Then, you do some method development using a silica TLC plate and see a major spot with some minor, early-eluting impurities. You think that the purification will be easy only to find that your “purified” compound has some co-eluting impurities. Now what? Should you change solvents or change stationary phase?

      In this post, I will show how changing the column media but keeping the same solvents removed a co-eluting impurity in one of my reaction mixes.

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      Determining solvent strength in flash column chromatography

      May 7, 2019 at 5:23 PM / by Bob Bickler posted in Developments, selectivity, solvent strength

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      Recently, one of our readers wrote and asked how to determine solvent strength in normal-phase flash chromatography. This is an excellent question because solvent strength is one of several factors impacting flash chromatography performance.

      In this post I will explain how solvent strength can easily be determined.

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