Welcome to the Biotage Flash Purification Blogs.

      The Step Gradient Explained

      November 1, 2019 at 9:37 PM / by Greg Saunders posted in Gradient, Selekt

      0 Comments

      Up to six compounds can be easily separated with an automated step-gradient optimizer embedded in modern flash chromatography systems.

      Read More

      How does solvent choice impact reversed-phase flash chromatography separations?

      October 4, 2019 at 6:30 PM / by Bob Bickler posted in Cannabis, Chromatography, Gradient, Normal phase, Optimization, SNAP, Solvents, Troubleshooting, Selekt, Media, Mass-directed purification, Theory

      0 Comments

      I have recently posted on how solvent choice influences the separation of hard to resolve compounds using normal-phase flash chromatography. As a chemist with an inquiring mind, I thought I would expand my research beyond normal-phase and see what happens in reversed-phase.

      In this post, I share my results. 

      Read More

      Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

      February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt, Media, Loading method

      0 Comments

      Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

      Read More

      Are there benefits to collecting flash chromatography fractions based on multiple UV wavelengths?

      January 8, 2019 at 7:29 PM / by Bob Bickler posted in Optimization, Selekt

      0 Comments

      UV detection and fractionation is ubiquitous in flash chromatography. It is the default methodology used to detect and collect eluting compounds. Today’s flash chromatography systems offer UV-triggered fractionation on one, two, or a range of wavelengths in order to either increase fractionation specificity, yield, or increase sensitivity.

      Read More

      The value of in-line mass detection with flash column chromatography

      December 11, 2018 at 7:56 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt

      0 Comments

      For many chemists, flash chromatography with UV-triggered fractionation is part of their everyday workflow. Prior to flash chromatography, the reaction mixtures are either analyzed by TLC, analyzed by LC-MS, or both to ensure the targeted product has been synthesized. But, what if the reaction created a lot of by-products? How do you find your product in a sea of impurities? In this post, I will discuss how using a flash purification system with an in-line mass detector will simplify flash purification and isolate the target molecule or molecules. 

      Read More