Welcome to the Biotage Flash Purification Blogs.

      Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

      February 5, 2019 at 8:10 PM / by Bob Bickler posted in dry load, silica, Flash column chromatography, sfär, Selekt

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      Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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      Are there benefits to collecting flash chromatography fractions based on multiple UV wavelengths?

      January 8, 2019 at 7:29 PM / by Bob Bickler posted in UV Detection, Selekt

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      UV detection and fractionation is ubiquitous in flash chromatography. It is the default methodology used to detect and collect eluting compounds. Today’s flash chromatography systems offer UV-triggered fractionation on one, two, or a range of wavelengths in order to either increase fractionation specificity, yield, or increase sensitivity. In this post, I will discuss the value of incorporating fractionation based on a wavelength range and two selectable individual wavelengths.

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