Welcome to the Biotage Flash Purification Blogs.

    The Step Gradient Explained

    November 1, 2019 at 9:37 PM / by Greg Saunders posted in Gradient, Selekt

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    Up to six compounds can be easily separated with an automated step-gradient optimizer embedded in modern flash chromatography systems.

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    Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

    February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt, Media, Loading method

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    Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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    Are there benefits to collecting flash chromatography fractions based on multiple UV wavelengths?

    January 8, 2019 at 7:29 PM / by Bob Bickler posted in Optimization, Selekt

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    UV detection and fractionation is ubiquitous in flash chromatography. It is the default methodology used to detect and collect eluting compounds. Today’s flash chromatography systems offer UV-triggered fractionation on one, two, or a range of wavelengths in order to either increase fractionation specificity, yield, or increase sensitivity.

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    The value of in-line mass detection with flash column chromatography

    December 11, 2018 at 7:56 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt

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    For many chemists, flash chromatography with UV-triggered fractionation is part of their everyday workflow. Prior to flash chromatography, the reaction mixtures are either analyzed by TLC, analyzed by LC-MS, or both to ensure the targeted product has been synthesized. But, what if the reaction created a lot of by-products? How do you find your product in a sea of impurities? In this post, I will discuss how using a flash purification system with an in-line mass detector will simplify flash purification and isolate the target molecule or molecules. 

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