Welcome to the Biotage Flash Purification Blogs.

      What are good reversed-phase dry load options?

      July 23, 2019 at 2:40 PM / by Bob Bickler posted in Reversed-phase, Sfär, Loading method

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      Using a “dry” loading technique with flash chromatography typically improves compound purity and overall separation quality compared to liquid loading. The reasons for this I have prophesized previously and include:

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      How many ways can you load sample on your column?

      May 31, 2019 at 4:02 PM / by Raffaella Bombarda posted in Chromatography, Samplet(R), Sfär, Media, Loading method

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      Biotage®, a pioneer in Flash Purification, launched the unique, removable cap SNAP flash chromatography columns in 2007. This beneficial column design feature continues with the newest Biotage flash columns named Sfär columns.

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      Does your crude sample/sorbent dry load ratio impact flash column chromatography results?

      February 5, 2019 at 8:10 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt, Media, Loading method

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      Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load sorbent impacts purification performance.

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      Dry loading vs. liquid loading, which provides better flash column chromatography results?

      January 23, 2019 at 2:15 PM / by Bob Bickler posted in Chromatography, Sfär, Media

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      When it comes time to purify your reaction mixture or natural product extract, you have a choice to make. Should you simply load your dissolved sample onto your flash column or take the extra step to adsorb your mix onto a sorbent and dry it before loading? The choice can have a major impact on your results.

      In this post, I will share results of work I conducted in my lab, comparing liquid and dry loading a reaction mixture that containing eight major components.

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      The value of in-line mass detection with flash column chromatography

      December 11, 2018 at 7:56 PM / by Bob Bickler posted in Chromatography, Sfär, Selekt

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      For many chemists, flash chromatography with UV-triggered fractionation is part of their everyday workflow. Prior to flash chromatography, the reaction mixtures are either analyzed by TLC, analyzed by LC-MS, or both to ensure the targeted product has been synthesized. But, what if the reaction created a lot of by-products? How do you find your product in a sea of impurities? In this post, I will discuss how using a flash purification system with an in-line mass detector will simplify flash purification and isolate the target molecule or molecules. 

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