Welcome to the Biotage Flash Purification Blogs.

      Which sorbents work best for dry loading flash column chromatography samples?

      October 18, 2019 at 10:05 PM / by Bob Bickler posted in dry load, solvent, media


      For chemists needing to purify natural product extracts or synthesis reaction mixtures flash chromatography is typically the tool of choice. In previous posts I have discussed various ways to optimize the purification to obtain the highest purity compounds with maximum load in minimal time.

      Sometimes, though, chemistry gets in the way in the form of solubility issues. When this happens most often dry loading is recommended for these sample types. In this post I will show the impact various dry load sorbent options have on chromatography.

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      How do I purify my high boiling solvent reaction mixture using silica flash chromatography?

      October 18, 2019 at 9:48 PM / by Bob Bickler posted in solvent, dissolve, purify, boiling point


      Many chemists today find they need to synthesize molecules at higher temperatures in order to force difficult reactions to proceed. Solvents such as DMF, DMSO, and NMP are commonly used in these reactions as they facilitate the use of the high reaction temperatures.  However, the same attributes that make these chemicals attractive as reaction solvents make compound recovery from them very difficult, including flash column chromatography.  These high boiling solvents are typically polar and pose a challenge if purification is to be accomplished with normal-phase silica.

      In this post I explore some options that enable purification of high boiling point solvated reaction mixtures through simple sample work-up.

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      Greener flash chromatography tips

      April 30, 2019 at 10:18 PM / by Bob Bickler posted in solvent, greener flash purification, particle size, surface area


      In our more environmentally aware climate, chemical and pharmaceutical companies now prioritize reducing organic solvent use in chemistry labs. Employees and shareholders alike are pushing their companies to become greener which impacts how chemistry, both synthesis and purification, is performed.

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