Welcome to the Biotage Flash Purification Blogs.

      How can I reduce flash column purification time and cost?

      May 8, 2019 at 2:57 PM / by Bob Bickler posted in efficiency, purity, speed


      This is a question being asked of my colleagues and me more and more frequently, especially in pharma accounts.  Why?  Well, you are familiar with the adage – Time is Money, right.  Well this really applies to them. A new molecular entity (NME) created as a pharmaceutical can take up to a decade and a billion dollars to bring to market.  Granted, the biggest costs are in the clinical trials but the synthetic route and the time to discover and make the compound – and purify it – plays a major role within drug discovery and development. This timeline is not helped by the ever increasingly difficult-to-synthesize compounds being investigated as drug candidates today.

      With that in mind, this post focuses on ways to speed the purification process without sacrificing purity and yield.

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      Flash column chromatography equilibration speed – how fast can you go?

      April 9, 2019 at 11:22 PM / by Bob Bickler posted in equilibration, speed, media


      Equilibrating silica flash chromatography columns is something I always do.  There are chemists who see this as an unnecessary, time-and-solvent-wasting step.  However, because getting consistent, predictable results is a priority for me, I equilibrate to remove the variability that can be caused by heat generated as solvent initially contacts the silica. Consistency is really important when running flash column chromatography because re-runs are time consuming and may put your compound at risk.

      In this post, I examine the role of equilibration speed and duration to show its impact, or lack there of, on purification performance.

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