Welcome to the Biotage Flash Purification Blogs.

      What is a Chromatography Gradient?

      October 2, 2019 at 3:27 PM / by Bob Bickler posted in isocratic, linear gradient, step-gradient, method development


      Gradients, used in chromatographic methods, assist with chemical separation and elution. They begin with “weak” elution conditions and end with “strong” elution conditions.

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      How do I Create an Efficient Gradient Flash Chromatography Method?

      September 20, 2019 at 2:58 PM / by Bob Bickler posted in linear gradient, step-gradient, method development


      For most organic and medicinal chemists flash chromatography is just another step in the synthesis work flow - react, analyze, purify, react, analyze, purify... until the final product is made. The desired product of each reaction, and the mixture of other species present are, of course, different with each cycle.  Separating the desired compound efficiently without a lot of hassle is something I have written about in this post as well as in others in this series.

      In this post, I've written about how that TLC (thin layer chromatography) plate you use for monitoring your reaction can be used to create reliable, efficient, effective gradients.

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      How can I rapidly remediate THC from CBD in my hemp extract using flash column chromatography?

      September 20, 2019 at 2:52 PM / by Bob Bickler posted in cannabis, CBD, hemp, linear gradient, remediate, remediation, reversed-phase, step-gradient, terpenes, THC, User Tips


      Tetrahydrocannabinol, aka THC, is a hallucinogen found in cannabis and, to a lesser degree, in hemp.  Though THC is legal in some locations in the US and Canada, there is a growing market for its non-hallucinogenic cousin, cannabidiol (CBD), which has purported medical benefits.

      The problem with isolating CBD from cannabis and hemp is contamination from THC, which is typically present at a moderate to high percentage. In this post, I will provide some insight into rapidly purifying CBD to remove THC.

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      How to Choose Between Linear Gradients and Step Gradients for Flash Chromatography

      April 12, 2019 at 12:51 PM / by Bob Bickler posted in Developments, efficiency, linear gradient, step-gradient


      Varying the concentrations of mobile phase solvents during  flash purification chromatography enhances the ability of the technique to effectively isolate the desired compound from reaction by-products and unconsumed reagents.  Choosing how these concentrations will be varied over time has a significant effect on the purity and recovery of desired compounds.

      What is the best starting strong solvent %?   What is the ending strong solvent %?  Should the mobile phase concentrations vary gradually in a linear manner or should they vary step-wise or something else altogether?  Most separations are performed once, occasionally a handful of times.  Because of this, spending effort optimizing a gradient is just not very productive unless there are aids in choosing the gradient profile that provides an effective purification with minimum effort.

      Software in flash chromatography instruments, makes it simple to create a gradient.  Now, what should that gradient look like?

      In this post I compare isocratic, step, and linear gradients and provide some sage advice on choosing among them.

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      The three reasons you should be using step gradients for flash column chromatography

      March 5, 2019 at 9:34 PM / by Bob Bickler posted in linear gradient, normal phase, step-gradient, greener flash purification


      For most synthesis and natural product chemists, flash chromatography is the primary tool for purification and isolation of compounds of interest. Purification methods include flash system defaulted linear gradients (e.g. 0-100%), active gradient modification (on-the-fly) during purification, and unique method creation based one either the chemist’s experience or TLC data (typically a linear gradient). Rarely do chemists work to optimize the purification to maximize target compound purity by employing a step gradient. In this post, I discuss the value optimized step gradients provide chemists.

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