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How to create a reversed-phase step gradient

February 21, 2023 at 4:01 PM / by Bob Bickler posted in reversed-phase, step gradient


Gradient elution in liquid chromatography (HPLC, flash chromatography) is the norm. There are two typical gradient types, the linear gradient, and the step gradient. Linear gradients are mostly used due to their relative simplicity but often lack the specificity needed to purify a specific compound to a desired percentage. That is where step gradient’s improved compound targeting can be beneficial. But how do you create a step gradient?

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