Welcome to the Biotage Flash Purification Blogs.

    Why is TLC Rf important for flash column chromatography optimization?

    December 28, 2020 at 4:10 PM / by Bob Bickler posted in Chromatography Fundamentals, TLC


    Many chemists I talk to understand that TLC data is useful for flash chromatography method development. Most also know that they should try to get their target compound to elute with an Rf between 0.15 and 0.4 by adjusting TLC solvent strength. Have you ever wondered why this is important and how Rf values impact flash chromatography results?

    In this post I will explain the relationship between TLC Rf and flash elution volumes (CV) and why having your target compound elute in the Rf range is needed.

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    Does high performance flash column chromatography require high performance TLC for method development?

    November 25, 2020 at 4:34 PM / by Bob Bickler posted in Chromatography Fundamentals, TLC


    Higher performance flash columns are becoming all the rage these days.   Chemists are using them for challenging as well as for routine purification.   As a result, I am often asked, "do I need high-performance TLC plates for method development?"

    In this post I will explain why the answer is no.

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    Invest 10 minutes on TLC and save a day of grief

    November 13, 2020 at 1:17 PM / by Bob Bickler posted in Chromatography Fundamentals, Green, TLC


    Flash column chromatography is used by between 20 and 40 thousand organic synthesis chemists worldwide, an amazing number. For most of these chemists flash chromatography is an important part of their daily workflow but allocating time for good method development is often not considered, which can lead to less than ideal purification results.

    In this post I focus on how allocating just 10 minutes on thin-layer chromatography (TLC) for method development can save you a lot of grief later on.

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