Welcome to the Biotage Flash Purification Blogs.

      How do I purify ionizable organic amine compounds using flash column chromatography?

      November 21, 2019 at 3:59 PM / by Bob Bickler posted in Amine, Chromatography, Gradient, Normal phase, Optimization, Polar, Solvents, Troubleshooting, TLC


      For most organic reaction mixture purifications the process is fairly straightforward. Use hexane/ethyl acetate or, for polar compounds, DCM/MeOH.  But what do you do if this doesn't work and your compounds are basic organic amines?

      In this post, I re-examine the options available to achieve an acceptable organic amine purification when typical separation methods are insufficient.

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      How many times can I reuse my flash chromatography column?

      November 21, 2019 at 3:40 PM / by Bob Bickler posted in Gradient, Normal phase, Optimization, Polar, Solvents, Troubleshooting


      Flash chromatography – a purification tool for both organic chemists and natural product researchers.  This tool is essential when you need to remove impurities from your targeted product, or products, in order to get them pure.  To reduce the costs associated with flash chromatography, some chemists try reusing the same column over and over, not always with success.

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      Why are my flash column chromatography peaks splitting?

      November 1, 2019 at 9:19 PM / by Bob Bickler posted in Troubleshooting


      Split peaks? Multiple peaks? Are they really a problem? What causes the issue?

      In this post I will discuss what split peaks are and what to do to fix the problem.

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      How does solvent choice impact flash column chromatography performance?

      October 18, 2019 at 9:44 PM / by Bob Bickler posted in Troubleshooting, Theory


      Selectivity and solvent strength are the most important factors that determine success or failure of a chromatographic separation. These two independent dynamics apply to both normal- and reversed-phase chromatography and should be optimized, especially when high fraction purity is needed.

      In this post I will discuss the impact that elution solvent choice has on both normal- and reversed-phase purification.

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      How does solvent choice impact reversed-phase flash chromatography separations?

      October 4, 2019 at 6:30 PM / by Bob Bickler posted in Cannabis, Chromatography, Gradient, Normal phase, Optimization, SNAP, Solvents, Troubleshooting, Selekt, Media, Mass-directed purification, Theory


      I have recently posted on how solvent choice influences the separation of hard to resolve compounds using normal-phase flash chromatography. As a chemist with an inquiring mind, I thought I would expand my research beyond normal-phase and see what happens in reversed-phase.

      In this post, I share my results. 

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      How can I rapidly remediate THC from CBD in my hemp extract using flash column chromatography?

      September 20, 2019 at 2:52 PM / by Bob Bickler posted in Cannabis, Gradient, Remediation, Reversed-phase, Troubleshooting


      Tetrahydrocannabinol, aka THC, is a psychoactive compound found in cannabis and, to a lesser degree, in hemp.  Though THC is legal in some locations in the US and Canada, there is a growing market for its non-hallucinogenic cousin, cannabidiol (CBD), which has purported medical benefits.

      The problem with isolating CBD from cannabis and hemp is contamination from THC, which is typically present at a moderate to high percentage. In this post, I will provide some insight into rapidly purifying CBD to remove THC.

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