Welcome to the Biotage Flash Purification Blogs.

      Why are my flash column chromatography peaks splitting?

      November 1, 2019 at 9:19 PM / by Bob Bickler posted in Troubleshooting


      Split peaks? Multiple peaks? Are they really a problem? What causes the issue?

      In this post I will discuss what split peaks are and what to do to fix the problem.

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      How does solvent choice impact flash column chromatography performance?

      October 18, 2019 at 9:44 PM / by Bob Bickler posted in Troubleshooting, Theory


      Selectivity and solvent strength are the most important factors that determine success or failure of a chromatographic separation. These two independent dynamics apply to both normal- and reversed-phase chromatography and should be optimized, especially when high fraction purity is needed.

      In this post I will discuss the impact that elution solvent choice has on both normal- and reversed-phase purification.

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      How does solvent choice impact reversed-phase flash chromatography separations?

      October 4, 2019 at 6:30 PM / by Bob Bickler posted in Cannabis, Chromatography, Gradient, Normal phase, Optimization, SNAP, Solvents, Troubleshooting, Selekt, Media, Mass-directed purification, Theory


      I have recently posted on how solvent choice influences the separation of hard to resolve compounds using normal-phase flash chromatography. As a chemist with an inquiring mind, I thought I would expand my research beyond normal-phase and see what happens in reversed-phase.

      In this post, I share my results. 

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      How can I rapidly remediate THC from CBD in my hemp extract using flash column chromatography?

      September 20, 2019 at 2:52 PM / by Bob Bickler posted in Cannabis, Gradient, Remediation, Reversed-phase, Troubleshooting


      Tetrahydrocannabinol, aka THC, is a hallucinogen found in cannabis and, to a lesser degree, in hemp.  Though THC is legal in some locations in the US and Canada, there is a growing market for its non-hallucinogenic cousin, cannabidiol (CBD), which has purported medical benefits.

      The problem with isolating CBD from cannabis and hemp is contamination from THC, which is typically present at a moderate to high percentage. In this post, I will provide some insight into rapidly purifying CBD to remove THC.

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