Welcome to the Biotage Flash Purification Blogs.

    Which solvents are best for terpene flash chromatography?

    July 6, 2022 at 2:59 PM / by Bob Bickler posted in UV, selectivity

    0 Comments

    Well, it’s spring as I write this, my favorite time of year, and the foliage is in various stages of bloom. I like the rebirth of nature and the floral aromas that emanate from the blooms. These botanical aromas are the result of the various terpenes and terpenoids in the plant which can be very different between species.

    Read More

    How to maximize UV detection sensitivity in flash chromatography

    June 21, 2022 at 4:11 PM / by Bob Bickler posted in Solvents, UV, wavelength, sensitvity

    0 Comments

    Automated flash chromatography has become an integral component of the workflows of both synthetic organic chemistry and natural product research. The most utilized chromatographic technique is normal-phase, which uses a polar stationary phase filled column (i.e. silica) and a mix of non-polar and moderately polar solvents, i.e. hexane or heptane and ethyl acetate (EtOAc), resp.

    Read More

    When do I need to use an ELSD with flash chromatography?

    May 11, 2022 at 2:16 PM / by Bob Bickler posted in Detector, UV, elsd

    0 Comments

    Flash chromatography can be complex. Solvent choice, column size, stationary phase, loading technique, gradient method, flow rate, and detection parameters are all variables which factor into flash chromatography and your success with this purification technique. Of these variables, detection parameters, i.e. the type of detector, can really impact your flash chromatography results.

    Read More

    How can flash chromatography UV absorbance spectra be used to identify purified compounds?

    August 25, 2020 at 1:52 PM / by Bob Bickler posted in Chromatography Fundamentals, UV, Spectra

    0 Comments

    As chemists, our job is to make new molecules. If our synthetic design works as planned, we will have success and the target product made. As we all know, along with our desired compound, by-products are often created as well. To remove the by-products, the reaction mixture requires purification, typically with flash chromatography.

    Read More