Welcome to the Biotage Flash Purification Blogs.

    How Can I Speed-up My Synthesis Workflow?

    October 20, 2020 at 2:04 PM / by Bob Bickler posted in Scavenger, V-10, Extraction method optimization, microwave, purification

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    Wouldn’t it be nice if your reactions only created your desired product? Of course the answer is yes, but that is not the reality of synthetic chemistry. Because our chemical reactions yield multiple components, they need work-up and purification to isolate the desired compound.

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    How do I remove an annoying MS TIC background?

    April 10, 2020 at 2:52 PM / by Bob Bickler posted in Chromatography Fundamentals, Troubleshooting and Optimization, Sfär, Detector, Normal Phase, V-10, Isolera

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    Have you ever run flash column chromatography with mass detection (Flash-MS) and observed the total ion current or TIC increase during the purification only to find that there was no discernible compound contributing to the effect?

    In this post I discuss how I came across this issue and the solution I found to work.

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    Organic Chemistry Workflow – Typical Steps and Equipment

    November 12, 2019 at 3:04 PM / by Bob Bickler posted in Workflow, Sfär, Selekt, Loading Techniques, Normal Phase, V-10, Pillar Page, Initiator

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    Synthetic organic chemistry is the genesis of new pharmaceutical and commercial chemical products. In short, it is based on the idea that two or more carbon-based compounds can be forced to react using heat, or other energy source, to create a new, novel product – but this we already know.

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    How do I purify my high boiling solvent reaction mixture using silica flash chromatography?

    October 18, 2019 at 9:48 PM / by Bob Bickler posted in Solvents, Troubleshooting and Optimization, Synthesis, V-10, Isolera, Initiator

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    Many chemists today find they need to synthesize molecules at higher temperatures in order to force difficult reactions to proceed. Solvents such as DMF, DMSO, and NMP are commonly used in these reactions as they facilitate the use of the high reaction temperatures.  However, the same attributes that make these chemicals attractive as reaction solvents make compound recovery from them very difficult, including flash column chromatography.  These high boiling solvents are typically polar and pose a challenge if purification is to be accomplished with normal-phase silica.

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    Preparation and use of an internal dry loading device for flash chromatography

    September 17, 2019 at 2:15 PM / by Bob Bickler posted in Sfär, Loading Techniques, V-10

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    Your choice of sample loading technique can, and likely will, affect the separation and purity of your targeted compound. While liquid loading is easy and often fit for purpose, it can provide some issues especially if large sample volumes are required relative to column size (> 1% of a column volume) or the dissolution solvent is too strong for the chosen purification method (e.g. injecting a methanol-solvated sample into a hexane/ethyl acetate mobile phase).

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    Let your worries evaporate...it's never been so easy!

    April 3, 2019 at 3:10 PM / by Raffaella Bombarda posted in Peptides, Solvents, Synthesis, Workflow, V-10, Pillar Page

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    Have you heard about Biotage® V-10 Touch? If you're struggling with rapidly drying samples dissolved in either aqueous or organic solvents, or evaporating HPLC fractions from purification and high boiling point solvents from synthesis, or simply if you would like to access a novel dry down onto silica technique for easier dry load capabilities, then you are in the right place! Just keep reading... 

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    Walk-up, Walk away Evaporation System

    April 3, 2019 at 2:59 PM / by Raffaella Bombarda posted in Solvents, V-10

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    Getting rid of solvent has long been a bottleneck in organic workflow.
    With the powerful Biotage® V-10 Touch evaporator, those days are gone.
    This system can evaporate DMSO and NMP with ease, meaning chemists
    are no longer limited to only volatile solvents in reaction design.

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    All you want to know about evaporation...and never dared to ask

    April 3, 2019 at 2:53 PM / by Raffaella Bombarda posted in Solvents, V-10

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    Biotage® V-10 Touch rapidly dries samples dissolved in both aqueous and organic solvents. It easily evaporates HPLC fractions from purification, high boiling point solvents from synthesis and features a novel dry-down onto silica technique for easier dry load capabilities prior to purification.

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