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    So, what exactly is a column volume in flash column chromatography and how is it determined?

    April 10, 2019 at 6:36 PM / by Bob Bickler posted in Chromatography, volume column


    In many of my previous posts I have used the term column volume, typically abbreviated as CV, as a value used to help determine separation quality and loading capacity.  However, I recently was asked a question about this topic from a chemist who understands the column volume concept but wanted to better understand its definition and how it is determined.

    In this post I will explain what a column volume is and how it is determined empirically.

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