Welcome to the Biotage Flash Purification Blogs.

    How to maximize UV detection sensitivity in flash chromatography

    June 21, 2022 at 4:11 PM / by Bob Bickler posted in Solvents, UV, wavelength, sensitvity

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    Automated flash chromatography has become an integral component of the workflows of both synthetic organic chemistry and natural product research. The most utilized chromatographic technique is normal-phase, which uses a polar stationary phase filled column (i.e. silica) and a mix of non-polar and moderately polar solvents, i.e. hexane or heptane and ethyl acetate (EtOAc), resp.

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    Does Detection Wavelength Influence Compound Purity and Recovery?

    June 29, 2021 at 7:09 PM / by Bob Bickler posted in purification, wavelength

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    Automated flash chromatography systems have helped synthetic chemists speed up their synthetic research. One major advancement with these systems over the past 15 or so years has been the addition of photo-diode array ultraviolet (PDA-UV) UV detectors with which chemists can detect and fractionate using one, two, or multiple wavelengths. Enabling detection and fractionation with multiple wavelengths increases the likelihood that target and by-product compounds will be isolated with increased purity.

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