Welcome to the Biotage Peptide Synthesis Blogs.

      What do you do when your peptide synthesis fails?

      Feb 14, 2019 2:40:48 PM / by Elizabeth Denton posted in peptide synthesis

      0 Comments

       It never fails. A sequence looks relatively straightforward on paper and it's of moderate length so you start synthesizing without too much thought into the protocol. Your synthesis is finished and you run the analytical HPLC but your product is nowhere to be found (or only very small quantities). So what went wrong? And what can you do differently to increase your crude yield and purity?

      Read More

      Synthesizing Disulfide-rich Peptides

      Feb 1, 2019 4:46:25 PM / by Elizabeth Denton posted in Disulfide Bonds, peptide synthesis, webinar

      0 Comments

      Elizabeth Denton, PhD, Senior Scientist Peptide Chemistry, recorded a webinar titled Disulfide Rich Peptides: Optimizing and Automating Syntheses with Regioselective Formation of Disulfide Bonds. To learn more, read the description below as well as watch the recording!

      Read More

      Synthesis of peptides containing three disulfide bonds: can it be fully automated?

      Jan 4, 2019 3:56:10 PM / by Elizabeth Denton posted in Peptides, Disulfide Bonds

      0 Comments

      I have experimented a lot with disulfide rich peptides lately, finding conditions that work well for not only the linear synthesis, but also for on-resin cysteine oxidations.  Although simple scaffolds are useful for determining orthogonal protecting group removal and cysteine oxidation conditions, many of the peptides of interest today are much more complex – three or more disulfide bonds, and often head-to-tail cyclization.

      In today’s post, I put to use three orthogonal protection strategies to optimize a fully automated synthesis of linaclotide.

      Read More