Welcome to the Biotage Peptide Synthesis Blogs.

      How St. John's University Accelerated their Cancer Research with the Biotage Peptide Workflow Solution

      Jan 11, 2019 4:14:31 PM / by Sarah Moran posted in Peptides, Synthesis, Workflow, Sfär, Customer case, Alstra, V-10, Isolera

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      "How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage Isolera, I could take it down to 10 or 15 minutes."

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      Synthesis of peptides containing three disulfide bonds: can it be fully automated?

      Jan 4, 2019 3:56:10 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Synthesis, Alstra

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      I have experimented a lot with disulfide rich peptides lately, finding conditions that work well for not only the linear synthesis, but also for on-resin cysteine oxidations.  Although simple scaffolds are useful for determining orthogonal protecting group removal and cysteine oxidation conditions, many of the peptides of interest today are much more complex – three or more disulfide bonds, and often head-to-tail cyclization.

      In today’s post, I put to use three orthogonal protection strategies to optimize a fully automated synthesis of linaclotide.

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