Let's be honest.  Optimizing a purification protocol for a single peptide is not something that one wants to spend a lot of time thinking about, let alone actually pursuing.  There are times though, that a little bit of effort in this arena can very impactful when considering the time required to fully synthesize, purify and deliver a peptide. 

In today's post, I'll discuss an approach combining the use of a focused gradient with a step gradient that minimizes the steps necessary to identify a more optimal flash purification gradient for peptide purification.