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      Peptide library synthesis: using two different coupling reagents to improve overall crude purity

      Jul 20, 2021 9:43:23 PM / by Elizabeth Denton posted in Automation, Method development, peptide synthesis


      Synthesizing libraries of peptides - potentially hundreds in a single synthesizer setup - has seen a resurgence of late.  These synthetic libraries, often prepared on very small synthesis scales, are required most recently for secondary screening preliminary structure-activity relationship determinations, or neoantigen vaccine preparations.  However, optimizing the synthesis of hundreds of peptides to be made simultaneously is a very different beast when compared to optimizing the synthesis of a single peptide.

      In today's post, I'll describe a strategy that can increase the overall crude purity of an entire peptide library using two different coupling chemistries.

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