Big pharmaceutical companies have begun to refocus their efforts towards peptide discovery projects with the hopes of identifying the next big peptide drug. There are often hundreds to thousands of peptides synthesized as part of these efforts, demanding parallel synthesis platforms and room temperature peptide synthesis protocols.
Previously, I identified a minimum number of amino acids equivalents required to ensure a high quality microwave synthesis. Conducting synthesis at room temperature will certainly require different conditions than microwave heating. Let’s explore how the number of equivalents will impact the synthesis results.