Every now and then I work with new groups as they embark on a journey incorporating peptides and peptide synthesis into their research. More often than not, no one in the group has experience with peptide synthesis as they are just getting operations off the ground. As a result, one of the most common questions I receive from these groups is how much amino acid should be used during synthesis.
In today’s post though I will address the number of equivalents of amino acid. Large numbers of amino acid equivalents can often be used to drive coupling reactions to near completion, but the question today is how few equivalents can be used to successfully synthesize your peptide.