Welcome to the Biotage Peptide Synthesis Blogs.

      How long are amino acid stock solution stable for successful solid phase peptide synthesis?

      Apr 29, 2019 6:03:52 PM / by Elizabeth Denton posted in peptide, peptide workflow, solid phase peptide synthesis, synthesis tips

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      In today’s post I’ll answer the above question by comparing the crude purity of peptides synthesized using amino acid stock solutions or freshly dissolved amino acids.

      In previous posts I have described using high concentrations of amino acids to improve your peptide synthesis among some other tips and tricks.  But there is a particularly handy tip that was left off the list.

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      Peptide Workflow by Biotage

      Mar 28, 2019 3:47:33 PM / by Elizabeth Denton posted in peptide, peptide synthesis, peptide workflow, remove solvents

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      What is the main goal of a peptide chemist? Elizabeth Denton, Ph.D., explains how Biotage sees the peptide synthesis workflow and how we focus on shortening the process time for scientists.

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      How to handle peptides that contain methionine

      Mar 28, 2019 2:24:34 PM / by Elizabeth Denton posted in Peptides, solid phase peptide synthesis, side reactions, methionine oxidation

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      The diversity of amino acid side chain functionalities, coupled with secondary structure, gives peptides and proteins their unique properties and activities. However, when it comes to chemically synthesizing peptides or even small proteins, the side chain functionalities can do more harm than good.

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      Why Evaporating DMSO is Not a Problem Anymore in Peptide Synthesis

      Mar 19, 2019 3:07:27 PM / by Sarah Moran posted in peptide workflow, v-10 touch, DMSO

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      Our seasoned peptide chemist Dr. Elizabeth Denton has tried it all when it comes to peptides. In this short video she shows that using DMSO (dimethyl sulfoxide) in a peptide synthesis workflow is perfectly fine together with the Biotage® V-10 Touch evaporator. Watch her evaporate it in just over eight minutes.

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      What do you do when your peptide synthesis fails?

      Feb 14, 2019 2:40:48 PM / by Elizabeth Denton posted in peptide synthesis

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       It never fails. A sequence looks relatively straightforward on paper and it's of moderate length so you start synthesizing without too much thought into the protocol. Your synthesis is finished and you run the analytical HPLC but your product is nowhere to be found (or only very small quantities). So what went wrong? And what can you do differently to increase your crude yield and purity?

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      Webinar: Synthesizing Disulfide-rich Peptides

      Feb 1, 2019 4:46:25 PM / by Elizabeth Denton posted in Disulfide Bonds, peptide synthesis, webinar

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      Elizabeth Denton, PhD, Senior Scientist Peptide Chemistry, recorded a webinar titled Disulfide Rich Peptides: Optimizing and Automating Syntheses with Regioselective Formation of Disulfide Bonds. To learn more, read the description below as well as watch the recording!

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      Synthesis of peptides containing three disulfide bonds: can it be fully automated?

      Jan 4, 2019 3:56:10 PM / by Elizabeth Denton posted in Peptides, Disulfide Bonds

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      I have experimented a lot with disulfide rich peptides lately, finding conditions that work well for not only the linear synthesis, but also for on-resin cysteine oxidations.  Although simple scaffolds are useful for determining orthogonal protecting group removal and cysteine oxidation conditions, many of the peptides of interest today are much more complex – three or more disulfide bonds, and often head-to-tail cyclization.

      In today’s post, I put to use three orthogonal protection strategies to optimize a fully automated synthesis of linaclotide.

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