Welcome to the Biotage Peptide Synthesis Blogs.

      Analyzing crude peptide samples by Mass Spectrometry: what are the options

      Jan 10, 2020 3:30:02 PM / by Elizabeth Denton posted in method development, peptide purification, analysis


      There are many techniques available to analyze and identify synthetic compounds that we are taught in the first few years of our chemistry education. While tools like NMR spectroscopy, IR spectroscopy, and others, are extremely useful for determining or confirming the structure of synthetic small molecules, these strategies are not as well suited for quick characterization of peptides. As a result, peptide chemists rely heavily on peak shape observed during a liquid chromatography step and mass spectrometry for mass confirmation.

      In today's post, I'll discuss several of the mass spectrometry techniques that are used for analyzing crude or purified peptide samples.

      Read More