Welcome to the Biotage Peptide Synthesis Blogs.

      Peptide library synthesis: using two different coupling reagents to improve overall crude purity

      Jul 20, 2021 9:43:23 PM / by Elizabeth Denton posted in Automation, Method development, peptide synthesis

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      Synthesizing libraries of peptides - potentially hundreds in a single synthesizer setup - has seen a resurgence of late.  These synthetic libraries, often prepared on very small synthesis scales, are required most recently for secondary screening preliminary structure-activity relationship determinations, or neoantigen vaccine preparations.  However, optimizing the synthesis of hundreds of peptides to be made simultaneously is a very different beast when compared to optimizing the synthesis of a single peptide.

      In today's post, I'll describe a strategy that can increase the overall crude purity of an entire peptide library using two different coupling chemistries.

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      Cyclic Peptides Webinar Recap

      Apr 15, 2020 2:40:31 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Webinar, Automation, Alstra

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      Peptides, while exhibiting potential for unique specificity and affinity, still suffer from stability issues when introduced to a biological system.  One strategy to overcome these stability issues is include a covalent bond, creating a peptide macrocycle.  

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      Does amino acid concentration really matter during peptide synthesis?

      Nov 8, 2019 4:15:44 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Media and Resin, Automation, Alstra

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      When it comes to synthesizing a peptide, the first thing that comes to mind is the number of stoichiometric equivalents to use.  Sometimes that number is as few as 1.5, sometimes it’s as high as 20!

      But have you ever thought about the liquid volume that contains those molecules and how that might affect the success of your coupling reaction?  In this post I will discuss the impact of amino acid concentration in the overall success of solid phase peptide synthesis.

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      How to handle peptides that contain methionine

      Mar 28, 2019 2:24:34 PM / by Elizabeth Denton posted in Peptides, Troubleshooting and Optimization, Synthesis, Automation, Alstra

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      The diversity of amino acid side chain functionalities, coupled with secondary structure, gives peptides and proteins their unique properties and activities. However, when it comes to chemically synthesizing peptides or even small proteins, the side chain functionalities can do more harm than good.

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