Peptides, while exhibiting potential for unique specificity and affinity, still suffer from stability issues when introduced to a biological system. One strategy to overcome these stability issues is include a covalent bond, creating a peptide macrocycle.
When it comes to synthesizing a peptide, the first thing that comes to mind is the number of stoichiometric equivalents to use. Sometimes that number is as few as 1.5, sometimes it’s as high as 20!
But have you ever thought about the liquid volume that contains those molecules and how that might affect the success of your coupling reaction? In this post I will discuss the impact of amino acid concentration in the overall success of solid phase peptide synthesis.
The diversity of amino acid side chain functionalities, coupled with secondary structure, gives peptides and proteins their unique properties and activities. However, when it comes to chemically synthesizing peptides or even small proteins, the side chain functionalities can do more harm than good.