Welcome to the Biotage Peptide Synthesis Blogs.

      What is solid phase peptide synthesis?

      Sep 25, 2019 9:47:21 PM / by Elizabeth Denton posted in Biotage, Developments, synthesis, peptide, peptide synthesis, solid phase peptide synthesis, cleavage, synthesis tips, orthogonal protecting groups, synthesis optimization, method development, FMOC, BOC


      More and more groups are exploring the utility of peptides with an ever widening variety of applications. And although peptides are getting cheaper to purchase outright, many groups are continuing to bring peptide synthesis in house. As more groups join the peptide community, I frequently encounter questions about the basics of peptide synthesis.

      In today's post, I'd like to cover a little history of solid phase synthesis as well as highlight some differences between the chemistries.

      Read More

      Peptides containing cysteine: the role of scavengers in cleavage cocktail

      Sep 11, 2019 10:48:03 PM / by Elizabeth Denton posted in peptide synthesis, cleavage

      1 Comment

      Since the development of Fmoc-based solid phase peptide synthesis, a wide variety of cleavage cocktails have emerged.  Each cleavage cocktail contains a unique combination of scavengers designed to prevent either side reactions mediated by the released protecting groups or the side chains themselves, or both during the peptide cleavage reaction.  As the number of scientists performing peptide synthesis grows, the question “which cleavage cocktail should I use?” comes up more often than not.

      In today’s post, I’ll highlight the role of of scavengers for peptides containing cysteine residues.

      Read More

      Post synthesis workup: What steps are necessary and what aren't?

      Jun 19, 2019 5:35:32 PM / by Elizabeth Denton posted in Developments, Peptides, workflow, peptide workflow, solid phase peptide synthesis, cleavage


      You’ve just finished a peptide synthesis and now it’s time to cleave the peptide from the resin. You’ve selected a specific cleavage cocktail, performed the reaction and now what? The vast majority of peptide chemists will precipitate their peptide using an ether solution, lyophilize, and move on to purification. But is that the only option?

      In today’s post I’ll highlight an alternative strategy that saves both processing time, potentially dangerous reagents, all without compromising the integrity of the recently synthesized peptide.

      Read More